Journal
VIROLOGY
Volume 346, Issue 1, Pages 229-237Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2005.11.002
Keywords
herpes; HSV; cell fusion; viral entry; glycoprotein B; mutation
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Herpes simplex virus (HSV) glycoprotein B (gB) is one of the four viral glycoproteins required for viral entry and cell fusion and is highly conserved among herposviruses. Mutants of HSV type 2 gB were generated by Substituting conserved residues in the cytoplasmic tail with alanine or by deleting 41 amino acids from the C-terminus. Some Of the Mutations abolished cell fusion activity and also prevented transport of gB to the Cell surface, identifying residues in the gB cytoplasmic tail that are critical for intracellular transport of this glycoprotein. These mutations also prevented production of infectious virus, possibly because the mutant forms of gB were not transported to the site of envelopment. Other mutations, particularly the deletion, significantly enhanced cell fusion activity. These mutations, as well as others described previously, identify regions of the gB cytoplasmic domain that modulate cell fusion activity. (c) 2005 Elsevier Inc. All rights reserved.
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