Journal
VIROLOGY
Volume 346, Issue 1, Pages 66-73Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2005.10.024
Keywords
Alfalfi mosaic virus; movement proteins; cell-to-cell movement; GFP; Ilarvirus; Bromovirus; Cucumovirus; Tobamovirus; Comovirus
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RNA 3 of Alfalfa mosaic virus (AMV) encodes the movement protein (MP) and coat protein (CP), Chimeric RNA 3 with the AMV MP gene replaced by the corresponding M P gene of Prunus necrotic ringspot virus, Brome mosaic virus, Cucumber mosaic virus or Cowpea mosaic virus efficiently moved from cell-to-cell only when the expressed MP was extended at its C-terminus with the C-terminal 44 amino acids of AMV MP. MP of Tobacco mosaic virus supported the movement of the chimeric RNA 3 whether or not the MP was extended with the C-terminal AMV MP sequence. The replacement of the CP gene in RNA 3 by a mutant gene encoding a CP defective in virion formation did not affect cell-to-cell transport of the chimera's with a functional MP. A GST pull-down technique was Used to demonstrate for the first time that the C-terminal 44 amino acids of the MP of a virus belonging to the family Bromoviridae interact specifically with AMV virus particles. Together, these results demonstrate that AMV RNA 3 can be transported from cell-to-cell by both tubule-forming and non-tuble-forming MPs if a specific MP-CP interaction occurs. (c) 2005 Elsevier Inc. All rights reserved.
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