Journal
PLANT AND CELL PHYSIOLOGY
Volume 47, Issue 3, Pages 426-431Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pci251
Keywords
Agrobacterium tumefaciens (Rhizobium radiobacter); functional genomics; Lycopersicon esculentum L.; Micro-Tom; transformation
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We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.
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