Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
Volume 42, Issue 2, Pages 144-147Publisher
SPRINGER
DOI: 10.1079/IVP2005743
Keywords
acclimatization; direct organogenesis; leaf explant; Populus ciliata; shoot regeneration
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This study reports a protocol for high-efficiency plant regeneration from leaf explants of male Himalayan poplar (Populus ciliata Wall.). Shoots were regenerated at high frequencies from explants grown on Murashige and Skoog (MS) medium supplemented with 0.5 mg l(-1) kinetin and 0.2 mg l(-1) indole-3-acetic acid (IAA). Regenerated shoots developed roots in MS medium supplemented with 0.1 mg l(-1) IAA. Himalayan poplar plantlets could be produced within 2 mo. after acclimatization in a sterile mixture of sand and soil.
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