Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1108, Issue 1, Pages 76-82Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2005.12.106
Keywords
oleuropein; OBPs; dynamic ultrasound-assisted extraction; polyphenols; olive leaves; biophenols
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A continuous approach for the ultrasound-assisted extraction of olive biophenols (OBPs) from olive leaves is proposed. Multivariate methodology was used to carry out a detailed optimisation of extraction. Under the optimal working conditions, complete extraction of the target analytes (namely, oleuropein, verbacoside, apigenin-7-glucoside and luteolin-7-glucoside with LODs 11.04, 2.68, 1.49 and 3.91 mg/kg, respectively) was achieved in 25 min. The extract was injected into a chromatograph-photodiode array detector assembly (HPLC-DAD) for individual separation-quantification. No clean-up or preconcentration steps were required. Gas chromatography-mass spectrometry (without derivatization of the analytes) was used to identify OBPs at concentrations below the LODs obtained by HPLC-DAD. The efficacy of ethanol-water mixtures to extract OBPs from olive leaves has been demonstrated and compared with that of a conventional method which requires 24 It for complete extraction; so these mixtures can substitute toxic extractants used to date. (c) 2006 Elsevier B.V. All rights reserved.
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