Novel method for preparation of β-cyclodextrin/grafted chitosan and it's application

A novel technique for preparation of P-cyclodextrin-grafted chitosan was carried Out by reacting P-cyclodextrin citrate (beta-CD citrate) with chitosan. beta-Cyclodextrin citrate was synthesis by esterifying beta-cyclodextrin (beta-CD) with citric acid (CA) it) presence or absence of sodium hypophosphite as a catalyst in a semidry process. Different factors affecting preparation of beta-CD citrates were Studied to obtain beta-CD citrate with high carboxyl content, Such factors include reaction temperature, citric acid concentration, material to liquor ratio and duration. beta-Cyclodextrin/grafted chitosan was prepared by coupling beta-CD citrate with chitosan dissolved in different formic acid solutions having different concentrations. The reacting ingredients were subjected to various reaction conditions to attain the optimum condition. beta-Cyclodextrin/grafted chitosan were evaluated by measuring the nitrogen content of both chitosan and grafted chitosan. Chitosan and l3-cyclodextrin/grafted chitosan, having different molecular weights, were evaluated as antimicrobial agents for different microorganisms Such as, Bacillus Megaterium, Pseudomonas Fragi, Bacillus Cereus Stophylococcus Aureus, Escherichia Ecoli and Aeromonas hydra. (c) 2005 Elsevier Ltd. All rights reserved.