Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 11, Pages 3972-3977Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0600285103
Keywords
adjuvant; glycolipid
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Natural killer T(NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with V alpha 14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl ceramide (GaIA-GSL) is a NKT cell agonist that is significantly weaker than alpha-galactosylceramicle (a-GalCer), the most potent known NKT agonist. For GaIA-GSL, a shorter fatty acyl chain, an absence of the 4-OH on the sphingosine tail and a 6'-COOH group on the galactose moiety account for its observed antigenic potency. We further determined the crystal structure of mCD1d in complex with GaIA-GSL at 1.8-A resolution. The overall binding mode of GaIA-GSL to mCD1d is similar to that of the short-chain a-GalCer ligand PBS-25, but its sphinganine chain is more deeply inserted into the F' pocket due to alternate hydrogen-bonding interactions between the sphinganine 3-OH with Asp-80. Subsequently, a slight lateral shift (> 1 A) of the galacturonosyl head group is noted at the CD1 surface compared with the galactose of a-GalCer. Because the relatively short C-14 fatty acid of GaIA-GSL does not fully occupy the A' pocket, a spacer lipid is found that stabilizes this pocket. The lipid spacer was identified by GC/MS as a mixture of saturated and monounsaturated palmitic acid (C-16). Comparison of available crystal structures of alpha-anomeric glycosphingolipids now sheds light on the structural basis of their differential antigenic potency and has led to the design and synthesis of NKT cell agonists with enhanced cell-based stimulatory activities compared with alpha-GalCer.
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