Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 6, Pages 1774-1779Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac051121q
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Funding
- NHLBI NIH HHS [R01 HL062244, R01 HL062244-05A1, R01 HL062244-06] Funding Source: Medline
- NIGMS NIH HHS [GM 038060, R01 GM038060, R01 GM038060-18, R01 GM038060-17] Funding Source: Medline
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Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium alpha-cyano-4-hydroxycinnamate and butylammonium 2,5dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.
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