Journal
VACCINE
Volume 24, Issue 12, Pages 2110-2119Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2005.11.026
Keywords
antigen 85A; dendritic cells; Mycobacterium tuberculosis
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In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4(+) and CD8(+) T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance. (c) 2005 Elsevier Ltd. All rights reserved.
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