Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 6, Pages 1841-1848Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac051868g
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Funding
- NCI NIH HHS [5P30 CA68485-07, P30 CA068485] Funding Source: Medline
- NIBIB NIH HHS [R33 EB000672, 1R21EB000672-01, R21 EB000672, R33 EB000672-03, R33 EB000672-04, 4R33EB000672-02] Funding Source: Medline
- NIEHS NIH HHS [P30 ES000267, 5P30 ES00267-36] Funding Source: Medline
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Surface plasmon resonance (SPR) has been used in determining kinetics and thermodynamics of biological interaction in the past decades. One difficulty encountered in this technology is the need for a proper regeneration, which means the removal of analytes from the bound complexes to regenerate the activity of the ligands. Regeneration is not always practical since the harsh regeneration reagents may destroy the bioactivity of the ligands. It is even more difficult for complexes with high affinity constants. In this paper, we report a nonregeneration protocol for SPR techniques in which subsequent ligand/analyte interactions can be measured without regeneration; thus ligand biological activity could be retained. Finetics, binding models, and mathematics of this protocol are discussed in detail using rabbit IgG as the analyte and engineered recombinant antibody A10B single-chain fragment variables (scFv) as the ligand. The affinity constant of rabbit IgG binding with A10B scFv measured by using a nonregeneration protocol was (2.5 +/- 0.2) x 10(7) M-1, which was comparable with the value determined with a conventional regeneration SPR method ((2.2 1.5) x 10(7) M-1) and quartz crystal microbalance (1.9 X 10(7) M-1). A paradigm of streptavidin-biotin binding was analyzed to validate this protocol. The affinity constant for each binding subunit of streptavidin to the immobilized biotin was determined to be (7.3 +/- 0.2) x 10(6) M-1, which was comparable with the solution-based value of 2 x 10(7) M-1. The nonregeneration protocol requires a relatively high ligand density on the biosensor surface so that more data points can be obtained before surface saturation. The small size of scFv enables them to be constructed in the biosensors for such purpose.
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