Journal
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM V
Volume 1137, Issue -, Pages 226-231Publisher
WILEY-BLACKWELL
DOI: 10.1196/annals.1448.009
Keywords
circulating DNA; cell-surface-bound DNA; hypermethylation; gastric cancer
Funding
- Russian Foundation for Basic Research [06-04-49732]
- Russian Academy of Sciences [13]
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The concentration of cell-free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence-based assay and methylation-specific PCR (MSP) in the blood of gastric cancer patients (n = 20) and healthy subjects (n = 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell-surface-bound DNA was not decreased compared with controls and amounted to 80 +/- 15% of the total circulating DNA. MSP analysis of three genes in the cell-surface-bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell-surface-bound DNA is a convenient source of DNA for MSP analysis of cancer-specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer-related changes in DNA can significantly contribute to cancer diagnostics.
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