Journal
JOURNAL OF NEUROSCIENCE
Volume 26, Issue 13, Pages 3465-3473Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4907-05.2006
Keywords
sodium channel; persistent sodium current; axon; excitability; layer 5 pyramidal neuron; neocortex
Categories
Ask authors/readers for more resources
In addition to the well described fast-inactivating component of the Na+ current [transient Na+ current (I-NaT)], neocortical neurons also exhibit a low-voltage-activated, slowly inactivating persistent Na+ current (INaP), which plays a role in determining neuronal excitability and synaptic integration. We investigated the Na+ channels responsible for I-NaP in layer 5 pyramidal cells using cell-attached and whole-cell recordings in neocortical slices. In simultaneous cell-attached and whole-cell somatic recordings, no persistent Na+ channel activity was detected at potentials at which whole-cell I-NaP operates. Detailed kinetic analysis of late Na+ channel activity in cell-attached patches at 36 C revealed that somatic Na+ channels do not demonstrate modal gating behavior and that the probability of single late openings is extremely low (< 1.4 X 10(-4) or < 0.02% of maximal open probability of I-NaT). Ensemble averages of these currents did not reveal a sustained component whose amplitude and voltage dependence could account for I-NaP as seen in whole-cell recordings. Local application of TTX to the axon blocked somatically recorded I-NaP, whereas somatic and dendritic application had little or no effect. Finally, simultaneous current-clamp recordings from soma and apical dendrite revealed that Na+ plateau potentials originate closer to the axon. Our data indicate that the primary source of I-NaP is in the spike initiation zone in the proximal axon. The focal axonal presence of regenerative subthreshold conductance with voltage and time dependence optimal to manipulate integration of synaptic input, spike threshold, and the pattern of repetitive firing provides the layer 5 pyramidal neuron with a mechanism for dynamic control of its gain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available