Journal
SCIENCE
Volume 311, Issue 5769, Pages 1940-1942Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1122975
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A characteristic feature of grasses and commercially important cereals is the presence of (1,3; 1,4)-beta-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3; 1,4)-beta-D-glucan content in barley grain to a cluster of cellulose synthase-like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3; 1,4)-beta-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3; 1,4)-beta-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3; 1,4)-beta-D-glucan biosynthesis.
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