Journal
OPTICS LETTERS
Volume 31, Issue 7, Pages 945-947Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.31.000945
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- NHLBI NIH HHS [P01HL64858] Funding Source: Medline
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Standing-wave total-internal-reflection fluorescence (SW-TIRF) microscopy uses a super-diffraction-limited standing evanescent wave to extract the high-spatial-frequency content of an object through a diffraction-limited optical imaging system. The effective point-spread function is better than a quarter of the emission wavelength. With a 1.45 numerical aperture objective and 532 nm excitation wavelength, a Rayleigh resolution of approximately 100 nm can be achieved, which is better than twice the resolution of conventional TIRF microscopy. This first experimental realization of SW-TIRF in an objective-launched geometry demonstrates the potential for extended resolution imaging at high speed by using wide-field microscopy. (c) 2006 Optical Society of America.
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