Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 4, Pages 793-800Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr050365o
Keywords
cysteine markers; electrochemistry; electrospray; mass spectrometry; protein structures probing
Categories
Ask authors/readers for more resources
The on-line electrochemical tagging (EC-tagging) of cysteine residues in proteins during mass spectrometry is studied to probe the cysteine environment. Benzoquinone probes electrogenerated at a microspray electrode react with the thiol functions of the proteins within a microchannel and the products are analyzed by mass spectrometry. The fundamentals of the technique are discussed, with a focus on the kinetic aspects. The EC-tagging efficiency of the cysteine residues in proteins is used to probe their environment. Experiments with unmodified proteins and their chemically reduced forms highlight the strong effect of the cysteine site reactivity on the tagging efficiencies. This study highlights relevant parameters for such on-line electrochemical derivatization/MS detection strategies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available