Journal
GENES & DEVELOPMENT
Volume 20, Issue 7, Pages 848-857Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1400206
Keywords
ChIP-chip; MSL complex; gene expression; chromatin modification; histone modification; dosage compensation
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Funding
- NIGMS NIH HHS [R37 GM045744, GM45744, R01 GM045744, K25 GM067825, GM67825] Funding Source: Medline
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X-chromosome dosage compensation in Drosophila requires the male-specific lethal (MSL) complex, which up-regulates gene expression from the single male X chromosome. Here, we define X-chromosome-specific MSL binding at high resolution in two male cell lines and in late-stage embryos. We find that the MSL complex is highly enriched over most expressed genes, with binding biased toward the 3 ' end of transcription units. The binding patterns are largely similar in the distinct cell types, with similar to 600 genes clearly bound in all three cases. Genes identified as clearly bound in one cell type and not in another indicate that attraction of MSL complex correlates with expression state. Thus, sequence alone is not sufficient to explain MSL targeting. We propose that the MSL complex recognizes most X-linked genes, but only in the context of chromatin factors or modifications indicative of active transcription. Distinguishing expressed genes from the bulk of the genome is likely to be an important function common to many chromatin organizing and modifying activities.
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