Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 8, Issue 4, Pages 615-620Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2006.02.005
Keywords
hemoglobin; cetylpyridinium bromide; thermodynamics; nitric oxide; electrochemical impedance spectroscopy
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Hemoglobin was successfully immobilized in cetylpyridinium bromide (CPB) film on a polyacrylamide (PAM) modified glassy carbon electrode (GC). Cyclic voltammetry showed a pair of well-defined and nearly reversible CV peaks at about -0.2165 V vs SCE (pH = 4.22), indicating the character of Hb heme Fe(II)/heme Fe(III) redox couple. The apparent standard potentials of hemoglobin in the CPB were linearly varied with pH in the range from 4.22 to 9.18 with a slope of -48.85 mV pH(-1), implying that one proton was accompanied with one electron transferred in the electrochemical reaction. The adsorption of Hb on the surface of PAM/GC electrode have been investigated by electrochemical impedance spectroscopy. The apparent standard potentials of hemoglobin were also studied through direct electrochemistry method as a function of temperature and the thermodynamic parameters (Delta S-rc and Delta H-rc) for the protein redox were calculated. Hemoglobin in the CPB exhibited catalytic activity for electrochemical reduction of NO. (c) 2006 Elsevier B.V. All rights reserved.
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