Journal
CYTOKINE
Volume 34, Issue 1-2, Pages 39-50Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2006.03.015
Keywords
TNF; IL-1; IKK; JNK; chromosomal breaks; cell cycle; caspases; IL-1b
Funding
- NIGMS NIH HHS [R01GM065325] Funding Source: Medline
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A role for pro-inflammatory cytokines in inflammation-related cancers has been suggested, but mechanisms are not defined. Here, we demonstrate that treatment of HeLa cells with TNF alpha increases chromosomal aberration. In contrast, IL-1 beta did not increase, but rather decreased chromosomal aberration. TNF alpha and IL-1 beta increased the production of H2O2 to similar levels in cells, suggesting that increased production of reactive oxygen species might not be the premier factor involved. Reducing H2O2 through overexpression of catalase or treatment of cells with NAC or BHA did not have an effect on TNF-induced chromosomal aberration. TNF alpha-induced NO production has been implicated in DNA damage. Inhibiting NO did not reduce TNF-induced chromosomal aberration. Inhibiting IKK, JNK, and p38 kinase as well as caspases decreased TNF-induced chromosomal aberration, and a correlation between TNF-induced apoptosis and CA generation was not found. Single-strand DNA breaks give rise to double-strand breaks, which then results in chromosomal breaks, when replication forks reach the single-strand breaks during S-phase. In cells progressing through S-phase, TNF alpha activation of IKK, JNK and p38 is significantly reduced. However, these kinases were activated by IL-1 beta in S-phase. The possibility that these pathways, in a TNF-specific manner.. may regulate either the generation of single- and double-strand breaks or their repair, thereby resulting in increased chromosomal aberration, is discussed. (c) 2006 Elsevier Ltd. All rights reserved.
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