Journal
JOURNAL OF BIOMOLECULAR SCREENING
Volume 11, Issue 3, Pages 247-252Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057105285610
Keywords
signal window; Z ' factor; assay variability ratio; assay validation
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In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They exanl ine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a Computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays.
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