Journal
ANALYTICAL BIOCHEMISTRY
Volume 351, Issue 1, Pages 18-25Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.01.038
Keywords
resouance light scattering; gold nanoparticles; cysteine
Ask authors/readers for more resources
The interaction between cysteine and gold nanoparticles was studied. Through the covalent combination with the -SH group and the electrostatic binding with the -NH3+ group of cysteine, gold nanoparticles call self-assemble to form a network Structure, Which results in greatly enhanced resonance light scattering (RLS). The experimental results demonstrate that the RLS technique offers a sensitive tool for investigations of self-assembly of nanoparticles. On the other hand, the RLS method can be applied to selectively determine cysteine with high sensitivity and simple operation. The linear range of determination of cysteine is from 0.01 to 0.25 mu g/mL with the detection limit of 2.0 ng/mL (16.5 nM, 3 sigma). None of the amino acids found in proteins inteferes with the determination. (c) 2006 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available