Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 384, Issue 7-8, Pages 1518-1524Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0309-5
Keywords
immunoglobulin G; gold nanoparticles; polyethylenimine; immunosensor
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A method based on use of functionalized gold nanoparticles on polyethylenimine film has been developed for colorimetric detection of immunoglobulin G (IgG). The immunogold nanoparticles were immobilized on quartz slides by recognition between antibody and antigen, with the antigen chemically adsorbed on the polyethylenimine film. By measurement of the UV-visible spectra of the immobilized immunogold, detection of h-IgG was achieved. The detection limit for h-IgG by use of this method can be as low as 0.01 mu g mL(-1). This method is quite promising for numerous applications in immunoassay.
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