Journal
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
Volume 128, Issue 2, Pages 271-278Publisher
ASME
DOI: 10.1115/1.2165689
Keywords
shear stress; cell deformability; cell adhesion; in vitro flow assay
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Funding
- NCI NIH HHS [R01 CA097306, R01 CA097306-01A1, R01 CA097306-03, R01 CA097306-02, CA-97306] Funding Source: Medline
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Experimental models that mimic the flow conditions in microcapillaries have suggested that the local shear stresses and shear rates can mediate tumor cell and leukocyte arrest on the endothelium and subsequent sustained adhesion. However further investigation has been. limited by the lack of experimental models that allow quantitative measurement of the hydrodynamic environment over adherent cells. The purpose of this study was to develop a system capable of acquiring quantitative flow profiles over adherent cells. By combining the techniques of side-view imaging and particle image velocimetry (PIV), an in vitro model was constructed that is capable of obtaining quantitative flow data over cells adhering to the endothelium. The velocity over an adherent leukocyte was measured and the shear rate was calculated under low and high upstream wall shear The microcapillary channel was modeled using computational fluid dynamics (CFD) and the calculated velocity profiles over cells under the low and high shear rates were compared to experimental results. The drag force applied to each cell by the fluid was then computed. This system provides a means for future study of the forces underlying adhesion by permitting characterization of the local hydrodynamic conditions over adherent cells.
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