Chemilummescence microfluidic chip fabricated in PMMA for determination of benzoyl peroxide in flour

A rapid and sensitive chemiluminescence microfluidic chip fabricated in polymethyl methacrylate for determination of benzoyl peroxide in flour is described. The width of the microchannel was 200 mu m and the depth was 100 pin in the chip (50 x 40 x 5 mm). The total length of the microchannel was 67 turn with the detection area of 5 x 5 mm. Benzoyl peroxide can directly oxidize luminol to produce chemiluminescence. Two injectors of double-tee injector and microvalve injector were used. The sampling volume was 0.16 mu l for the double-tee injector and 0.25 mu l for the microvalve injector. The linear range of the benzoyl peroxide concentration was 8 X 10(-7)-1 X 10(-4) g/ml. The detection limit was 4 x 10(-7) g/ml and the R.S.D for eight times is 3.06% for the double-tee injector and 2.41% for the microvalve injector. The proposed method has been successfully applied to the determination of benzoyl peroxide in flour. (c) 2005 Elsevier Ltd. All rights reserved.