Journal
ARCHIVES OF VIROLOGY
Volume 151, Issue 4, Pages 819-826Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-005-0654-2
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00001003, BBS/E/I/00001191] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00001191, BBS/E/I/00001003] Funding Source: Medline
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A nested PCR assay, with an internal control, was developed to detect African swine fever virus (ASFV) DNA in Ornithodoros erraticus. The assay revealed a better analytical sensitivity than virus isolation and the OIE PCR protocol. All ticks collected from the field, which were positive by virus isolation, were also positive by PCR. Viral DNA was detected in a further 19 out of 60 ticks from which no virus was isolated. Our results show that this assay is reliable and can easily be used to screen large tick populations collected in the field for the presence of ASFV.
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