Journal
PHYSIOLOGY
Volume 21, Issue -, Pages 86-92Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiol.00057.2005
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cAMP is an important second messenger with a plethora of cellular effects and bio-logical roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleoticle-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.
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