Journal
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
Volume 2, Issue 2, Pages 313-323Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1517/17425255.2.2.313
Keywords
biological oxygen consumption; mechanisms of toxicity; mitochondrial function; optical oxygen sensing; phosphorescent oxygen probes; respirometric assays; screening
Ask authors/readers for more resources
Analysis of mitochondrial dysfunction is of particular importance in drug development, as it has been implicated in many common diseases and therapeutic treatments. Here, the markers of mitochondrial function and toxicity are reviewed, as well as current methods of assessment, with particular emphasis on oxygen respirometry. Fluorescence-based Respirometric Screening Technology (RST) allows convenient high-throughput analysis of oxygen consumption by cells, isolated mitochondria, enzymes, tissues and organisms, and is, therefore, of high value for such studies and general absorption, distribution, metabolism and excretion and toxicology studies. Various RST assay formats are described and specific applications are discussed. Consideration is also given to the future potential of this analytical approach.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available