Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 50, Issue 4, Pages 1522-1524Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.50.4.1522-1524.2006
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The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.
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