Journal
NATURE METHODS
Volume 3, Issue 4, Pages 263-265Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH864
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We have developed a single-plasmid system for the efficient bacterial, expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNA(CUA)(Tyr)) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.
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