Journal
JOURNAL OF LIPID RESEARCH
Volume 47, Issue 4, Pages 804-814Publisher
ELSEVIER
DOI: 10.1194/jlr.M500506-JLR200
Keywords
low density lipoprotein; intermediate density lipoprotein; normal-phase high-performance liquid chromatography-mass; spectrometry; reversed-phase liquid chromatography-tandem mass spectrometry familial hypobetalipoproteinemia
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A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used first for class separation, followed by reversedphase LC-MS or LC-tandem mass spectrometry using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species and showed clear differences between lipids associated with apolipoprotein B-100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B-67-containing lipoproteins, isolated from a homozygote patient with familial hypobeta-lipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.
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