Journal
BIOTECHNOLOGY LETTERS
Volume 28, Issue 8, Pages 559-565Publisher
SPRINGER
DOI: 10.1007/s10529-006-0017-4
Keywords
carbon paste electrode; electrochemical assay; alpha-1-fetoprotein; magnetic nanoparticles
Categories
Ask authors/readers for more resources
A direct electrochemical immunoassay system based on the immobilization of alpha-1-fetoprotein antibody (anti-AFP), as a model system, on the surface of core-shell Fe2O3/Au magnetic nanoparticles (MNP) has been demonstrated. To fabricate such an assay system, anti-AFP was initially covalently immobilized on to the surface of core-shell Fe2O3/Au MNP. Anti-AFP-modified MNP (bio-nanoparticles) were then attached to the surface of carbon paste electrode with the aid of a permanent magnet. The performance and factors influencing the performance of the resulting immunosensor were studied. alpha-1-Fetoprotein antigen was directly determined by the change in current or potential before and after the antigen-antibody reaction versus saturated calomel electrode. The electrochemical immunoassay system reached 95% of steady-state potential within 2 min and had a sensitivity of 25.8 mV. The linear range for AFP determination was from 1 to 80 ng AFP ml(-1) with a detection limit of 0.5 ng AFP ml(-1). Moreover, the direct electrochemical immunoassay system, based on a functional MNP, can be developed further for DNA sensor and enzyme biosensor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available