Journal
HUMAN AND ECOLOGICAL RISK ASSESSMENT
Volume 12, Issue 2, Pages 248-258Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10807030500531539
Keywords
aerobic; biochemical pathway; biodegradation; mangrove; metabolites; 3-methylindole
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3-Methylindole (3MI), an N-heterocyclic aromatic compound also called skatole, is associated with animal waste and industrial processing. A pure culture of bacterium capable of using 3MI as the sole source of carbon and energy was isolated from mangrove sediment using an enrichment technique and identified as Pseudomonas aeruginosa Gs based on 16S rDNA sequence. Microbial degradation of 3MI was studied in batch culture experiments for several factors, including initial substrate concentrations, pH, and salinity. The optimum pH and salinity was 7.0 and 5 parts per thousand, respectively. Degradation of 3MI by P aeruginosa Gs was quantified by reversed-phase high-performance liquid chromatography. Two metabolites of 3MI degradation were detected and proposed to be indoline-3-carboxylic acid and indoline-3-ol based on data obtained from HPLC/MS. Our results suggest that 3MI call be rapidly degraded by indigenous microorganisms found in mangrove sediment.
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