Journal
JOURNAL OF CELL BIOLOGY
Volume 173, Issue 1, Pages 121-132Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200509076
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Funding
- NIAID NIH HHS [K08 AI055602-03, R01 AI049524, AI55602, K08 AI055602, K08 AI055602-02, AI49524, K08 AI055602-01] Funding Source: Medline
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The tumor natural killer (NK) cell line YTS was used to examine the cytoskeletal rearrangements required for cytolysis. A multiprotein complex weighing similar to 1.3 mD and consisting of WASp-interacting protein (WIP), Wiskott-Aldrich syndrome protein (WASp), actin, and myosin IIA that formed during NK cell activation was identified. After induction of an inhibitory signal, the recruitment of actin and myosin IIA to a constitutive WIP-WASp complex was greatly decreased. Both actin and myosin IIA were recruited to WIP in the absence of WASp. This recruitment correlated with increased WIP phosphorylation, which was mediated by PKC theta. Furthermore, the disruption of WIP expression by WIP RNA interference prevented the formation of this protein complex and led to almost complete inhibition of cytotoxic activity. Thus, the multiprotein complex is important for NK cell function, killer cell immunoglobulin-like receptor inhibitory signaling affects proteins involved in cytoskeletal rearrangements, and WIP plays a central role in the formation of the complex and in the regulation of NK cell activity.
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