Journal
SCIENCE
Volume 312, Issue 5771, Pages 269-272Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1123191
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- NICHD NIH HHS [HD047013] Funding Source: Medline
- NIDDK NIH HHS [DK065283, DK036054] Funding Source: Medline
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Gene transcription may be regulated by remote enhancer or insulator regions through chromosome looping. Using a modification of chromosome conformation capture (3C) and fluorescence in situ hybridization, we found that one allele of the insulin-like growth factor 2 (Igf2)/H19 imprinting control region (ICR) on chromosome 7 colocalized with one allele of Wsb1/Nf1 on chromosome 11. Omission of CCCTC-binding factor ( CTCF) or deletion of the maternal ICR abrogated this association and altered Wsb1/Nf1 gene expression. These findings demonstrate that CTCF mediates an interchromosomal association, perhaps by directing distant DNA segments to a common transcription factory, and the data provide a model for long-range allele-specific associations between gene regions on different chromosomes that suggest a framework for DNA recombination and RNA trans-splicing.
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