Journal
JOURNAL OF IMMUNOLOGY
Volume 176, Issue 8, Pages 4706-4715Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.176.8.4706
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Memory B cells help to protect the host from invading pathogens by maintaining persistent levels of Ag-specific serum Ab and generating rapid Ab responses upon re-exposure to Ag. Unambiguous identification of memory B cells has been a major obstacle to furthering our knowledge concerning both the development of B cell memory and secondary Ab responses due to an absence of specific cell surface markers. Germinal centers (GCs) are thought to be the major site of Ig hypermutation and Ag-driven selection of memory B cells. To develop a model that would identify GC-derived memory B cells, we generated transgenic mice that expressed cre recombinase in a GC-specific fashion. Interbreeding these mice with the cre-reporter strain, ROSA26R, produced progeny in which beta-galactosidase (beta-gal) was permanently expressed in B cells of the GC-memory pathway. Analysis following immunization with (4-hydroxy-3-nitrophenyl)acetyl coupled to chicken gamma globulin showed that long-lived beta-gal(+) B cells exclusively contained somatically mutated lambda(1) V regions and were capable of producing Ag-specific Ab-forming cell (AFC) responses that were > 100-fold higher than those afforded by beta-gal(-) B cells following adoptive transfer to naive hosts. Secondary challenge of immune mice showed that only similar to 20% of secondary AFCs expressed beta-gal. Interestingly, we found that somatic hypermutation of rearranged lambda(1) V regions within secondary AFCs showed a strong correlation with beta-gal expression, suggesting that nonmutated B cells contribute significantly to secondary Ab responses. This model should provide useful insights into memory B cell development, maintenance, and differentiation following immunization or pathogenic infection.
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