Journal
ANALYTICAL BIOCHEMISTRY
Volume 351, Issue 2, Pages 193-200Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.01.007
Keywords
quantum dots; silica; encoded bead; multiplex assay
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Novel -COOH modified polystyrene beads were prepared by sulfonation grafting, and the Surface area and pore Volume are greatly improved in comparison with the swelling-treated beads. The optimization coating time is 4 h, and the corresponding -COOH content is approximately 2.1 mmol/g. The scanning electron microscope results show that the silica particles deposited oil the beads and formed a silica shell that decreases the leakage of quantum dots (QDs) preferably and improves the bar code stability greatly. The anti-photo-bleaching of silica-coated beads was studied systemically, and the results show that the half-decay time (t(1/2)) of the coated beads increases to 537 s-seven times longer than that of the uncoated ones. Further DNA probe hybridization experiments indicated that the coding signal and target signal can be detected simultaneously and that the assays based oil these probe-conjugated silica/QD/polystyrene beads have good specificity and sensitivity that can detect a concentration as low as 0.01 mu g/ml target DNA in denatured calf thymus DNA solution, indicating that it is feasible to use this kind of bead for multiplex analysis. (c) 2006 Elsevier Inc. All rights reserved.
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