Journal
BIOSENSORS & BIOELECTRONICS
Volume 21, Issue 10, Pages 1987-1990Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2005.09.010
Keywords
SPR; CRP; immunosensor; inflammation; antiCRP clone C2 and C6
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The c-reactive protein (CRP) is one of the significant human blood serum markers for inflammatory processes. The serum presence of this hepatic similar to 115 kDa protein of five identical subunits accompanies several diseases (e.g.. CVD, inflammatory bowel diseases) and is nowadays detected by high-sensitivity ELISA assays in blood serum. To enable CRP detection in other matrices, an SPR-based (surface plasmon resonance) immunosensor for the CRP detection has been established. A linear detection range of 2-5 mu g CRP per ml was found, using two different antiCRP antibodies (monoclonal, IgG) for CRP trapment and detection. Furthermore, the kinetic antibody association and dissociation constants of one antibody (antiCRP, clone C2) could be determined. (c) 2005 Elsevier B.V. All rights reserved.
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