Journal
FEBS LETTERS
Volume 580, Issue 9, Pages 2365-2370Publisher
WILEY
DOI: 10.1016/j.febslet.2006.03.054
Keywords
thrombospondin-1; knockdown; RNAi; cellular infection; human coronary artery smooth muscle cell; Trypanosoma cruzi
Funding
- NCRR NIH HHS [RR 003032] Funding Source: Medline
- NHLBI NIH HHS [HL 007737] Funding Source: Medline
- NIAID NIH HHS [AI07281, AI 056667] Funding Source: Medline
- NIGMS NIH HHS [GM08037] Funding Source: Medline
- NIMHD NIH HHS [MD 000551] Funding Source: Medline
Ask authors/readers for more resources
Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transcripts and protein expression in mammalian cells causing inhibition of T. cruzi infection. Addition of TSP-1 to these cells restores infection. Thus, host TSP-1, regulated by the parasite, plays a crucial role in early infection. This is the first report showing that a human parasite modulates TSP-1 expression to facilitate infection. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available