Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 20, Issue 5, Pages 429-433Publisher
WILEY
DOI: 10.1002/bmc.579
Keywords
anabolic steroids; stanozolol; 3 '-hydroxystanozolol; gestrinone; 17 alpha-trenbolone; doping analysis
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A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. ne LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method. Copyright (c) 2005 John Wiley & Sons, Ltd.
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