Journal
SOIL BIOLOGY & BIOCHEMISTRY
Volume 38, Issue 5, Pages 1162-1165Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2005.07.015
Keywords
DNA fingerprinting; genetic diversity; host preference; clover
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We compared trapping of Rhizobium leguminosarum biovar trifolii isolates under field soil and non-soil environmental conditions. Isolates were obtained from white clover (Trifolium repens L.) and red clover (T. pratense L.) grown directly in the field and from plants inoculated with soil from the same site using a plant infection technique. Isolates were identified by genomic polymerase chain reaction (PCR) fingerprinting using primers derived from the enterobacterial repetitive intergenic consensus (ERIC) sequences. The isolates trapped from soil dilutions in the laboratory included a number of major ERIC types that were not found in the field trapped nodules, suggesting that sampling of clover Rhizobium strains from soil dilutions may not be representative of the field nodulating population. (c) 2005 Elsevier Ltd. All rights reserved.
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