Journal
PROTEOMICS
Volume 6, Issue 10, Pages 3109-3113Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200500717
Keywords
affinity chromatography; biomarker; 2-DE; non-human primate; serum
Funding
- NIDA NIH HHS [R01 DA 013770] Funding Source: Medline
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Non-human primates are an important biomedical research model organism and offer great promise for serum biomarker proteomic studies. However, potential obstacles to these studies include affinity serum depletion methods based on human antigens, depletion methods altering quantitation, and incomplete non-human primate genome sequences for protein identification. In the present study, high-abundance protein removal from monkey serum using a human multiple affinity removal system (MARS) was shown to be specific and did not alter quantitation. Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins.
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