Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 11, Issue 3, Pages -Publisher
SPIE-INT SOCIETY OPTICAL ENGINEERING
DOI: 10.1117/1.2204029
Keywords
urokinase-type plasminogen activator; protease; optical imaging; fluorescence; tumor
Funding
- NCI NIH HHS [R01-CA99385, P50-CA86355] Funding Source: Medline
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The ability to image tumor associated protease in vivo has biological and clinical implications. In the present study, we describe the development and validation of a urokinase-type plasminogen activator (uPA) sensitive fluorescence imaging probe. The activation of our probe is highly specific to uPA in both enzymatic and cellular-based assays. In two distinct in-vivo tumor models (human colon adenocarcinoma HT-29 and human fibrosarcoma HT-1080), the observed fluorescence changes correlate well with tumor associated uPA activity. The signal intensities of the tumors are about three-fold higher in animals with probe injections. Our results suggest a direct detection method for uPA activity in vivo and the approach can be used for monitoring tumor growth and development. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
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