Journal
ANNALS OF PLASTIC SURGERY
Volume 61, Issue 5, Pages 566-571Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SAP.0b013e31816d9579
Keywords
adipose tissue engineering; electrospinning; nanofibers; mesechymal stem cells; tissue engineering
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Funding
- Intramural Research Program of NIAMS [NIH Z01 AR 41131]
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Electrospun 3-dimensional nanofibrous scaffolds share morphologic similarities to collagen fibrils, and promote favorable biologic responses of seeded cells. In this Study, we have fabricated a 3-dimensional nanofibrous scaffold made of poly L-lactic acid, and examined its ability to support and maintain the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro. After a 21-day incubation, on red O staining of constructs treated with adipogenic supplements revealed positive adipose-like staining, compared with lack of staining in untreated cultures. Semi-quantitative RT-PCR analysis of human bone marrow-derived mesenchymal stern Cells Cultured in adipogenic medium revealed highly elevated levels of adipogenesis-associated genes (1797-fold for lipoprotein lipase, and 5.6-fold for peroxisome proliferator-activated receptor gamma). Immunofluorescence staining of cellular constructs in adipogenic culture media showed the presence of lipoprotein lipase vesicles, a characteristic feature of adipose tissue. These results suggest that the poly L-lactic acid-based nanofibrous scaffold is a promising candidate for adult stern cell-based engineering of adipose tissue.
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