Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 36, Issue 5, Pages 1093-1103Publisher
WILEY
DOI: 10.1002/eji.200535637
Keywords
cell differentiation; costimulation; cytokine receptors; graft versus host disease; tolerance
Categories
Funding
- NCI NIH HHS [CA81383] Funding Source: Medline
- NHLBI NIH HHS [T32-HL007781] Funding Source: Medline
- NIAID NIH HHS [AI50823] Funding Source: Medline
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Naive, CD4(+) T cells proliferate extensively but fail to differentiate when they are transferred into unirradiated recipients that express alloantigen or transgenic antigen on all MHC class II+ cells. Addition of an agonist antibody to OX40 (CD134), a costimulatory TNF receptor family member expressed on activated CD4+ T cells, enables the proliferating T cells to accumulate as differentiated effector cells and kill the host animals. The donor T cells from anti-OX40-treated animals express high levels of IL-2R alpha (CD25) and acquire the ability to secrete IFN-gamma when stimulated with IL-12 and IL-18. OX40 promotes differentiation by 48 h in T cell priming, before changes in Bcl-2 expression or cell recovery become apparent. We found that a Bcl-2 transgene or deficiency in Fas or TNFR1 failed to influence accumulation of differentiated donor cells, and found larger changes in expression of cytokine and cytokine receptor genes than in survival genes. Accumulation of differentiated CD4(+) effector T cells is initiated directly through OX40, but some OX40-deficient donor cells can gain effector function as bystanders to OX40(+/+) cells. Taken together, these data suggest that CD4(+) T cell differentiation to effector function is an important effect of OX40 engagement under conditions of ubiquitous antigen presentation.
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