Maskless liquid-crystal-display projection photolithography for improved design flexibility of cellular micropatterns

We previously developed all all-in-one photopolymerization device by modifying a commercially available liquid crystal display projector (LCDP) for the preparation of micropatterned surfaces and microfluidic channels Without the need for expensive photomasks. In the present Study, we demonstrate a simple and reliable method for rapid prototyping of cell micropatterning with high resolution using the modified LCDP device. Fabrication of complicated and flexible patterns was achieved using this device with positive-type photoresist ill a two-step process. First, micropatterns oil the silanized coverslips were fabricated from positive photoresist. Second, acrylamide monomer solution containing polymerization initiator wits dropped onto the micropatterned positive photoresist and copolymerized on the silanized coverslips ill situ by thermally initiated radical polymerization. After the reaction, the remnant micropatterned photoresist is easily dissolved, resulting in it polyacrylamide-silane micropattern oil the coverslip. The resultant polyacrylamide layer is highly hydrophilic and repels both protein adsorption and cell adhesion. Cells seeded oil the micropatterned surfaces therefore attach and spread only oil unpolymerized silanized glass surfaces, conforming to the pattern design. This technique is therefore useful for inexpensive, rapid prototyping of surface micropatterns using polymer materials. (c) 2006 Elsevier Ltd. All rights reserved.