Journal
MYCOLOGIA
Volume 98, Issue 3, Pages 514-517Publisher
ALLEN PRESS INC
DOI: 10.3852/mycologia.98.3.514
Keywords
AFLP; oomycetes; reverse genetics; TILLING
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We present a strategy to recover high molecular weight genomic DNA from large numbers of isolates of Phytophthora. Included are steps for generating mycelial mass in 24-well reuseable deep well plates, efficient lyophilization and disruption of the mycelium and genomic DNA extraction with 96-well glass fiber filter plates. The resulting DNA is consistently high molecular weight and is suitable for applications that require high quality DNA such as AFLP analysis and TILLING. A single operator easily can manage mycelium preparation and/or DNA extraction from 384 isolates in a single day and this approach might be useful for other fungi or fungi-like organisms that can be grown in liquid media.
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