Journal
CARBOHYDRATE RESEARCH
Volume 341, Issue 6, Pages 782-785Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2006.01.008
Keywords
degree of acetylation; chitin; chitosan; UV
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Determination of the degree of acetylation of chitosan by UV spectrophotometry using dual standards is investigated. The UV absorbance of a pure chitosan solution is contributed additively by the N-acetylglucosamine and glucosamine residues; the absorbance divided by the total molar concentration of the residues (A/c(1)) is linearly related to the degree of acetylation (DA). Using acetyl glucosamine and glucosamine hydrochloride as standards in 0.1 M hydrochloric acid solution, the equation obtained by linear regression is A/c(1) = 3.3615DA + 0.0218, R-2 = 0.9958. The DA of the analytical sample (in milligram of chitosan in V liters solution) can be calculated by DA = (3.361m-42.1 . A . V)/(161.1 . A . V - 0.218m) (c) 2006 Elsevier Ltd. All rights reserved.
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