Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 9, Pages 3084-3089Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0519460
Keywords
-
Categories
Funding
- NHGRI NIH HHS [1R01 HG002644-01A1] Funding Source: Medline
- NIGMS NIH HHS [T32 GM07616] Funding Source: Medline
Ask authors/readers for more resources
Single-cell gene expression analysis holds great promise for studying diverse biological systems, but methodology to process these precious samples in a reproducible, quantitative, and parallel fashion remains challenging. Here, we utilize microfluidics to isolate picogram and subpicogram mRNA templates, as well as to synthesize cDNA from these templates. We demonstrate single-cell mRNA isolation and cDNA synthesis, provide quantitative calibrations for each step in the process, and measure gene expression in individual cells. The techniques presented here form the foundation for highly parallel single-cell gene expression studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available