Journal
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
Volume 42, Issue 3, Pages 326-330Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683806030185
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The experimental conditions for the sulfation of legume galactomannans were found, which allow for the obtainment of polysaccharides with a high degree of substitution. Sulfate esters of four galactomannans of different composition (galactose content, 16.4-47.5%) were synthesized using the SO3-pyridine complex in dimethylformamide as a sulfating agent. The degree of substitution was as high as 1.4-1.8; it did not correlate with the content of galactose in the poly saccharides. It was found that the degree of sulfation depended on the reaction temperature in the range of 19-60 degrees C.
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