Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 5, Pages 1267-1275Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060015h
Keywords
mouse brain; prefractionation; MicroSol-IEF; solution isoelectricfocusing; mass spectrometry; MALDI; two-dimensional gel electrophoresis
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Analyzing complex protein mixtures on a single gel does not allow separation of many extracted proteins. Herein, we tried a prefractionation approach and mouse brain proteins were separated on a narrow pH range ZOOM-IEF Fractionator (MicroSol-IEF device) and run on two-dimensional gel electrophoresis. A total number of 2673 protein spots including 255 hydrophobic structures were successfully analyzed by mass spectrometry. This nonsophisticated approach to increase protein identification of a brain protein extract is a step forward in neurochemistry.
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