Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 92, Issue 1, Pages 45-49Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2006.02.007
Keywords
phage-display; cry-toxins; insect specificity
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Funding
- NIAID NIH HHS [1R01 AI066014-01] Funding Source: Medline
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The Cry1Ac toxin from Bacillus thuringiensis was displayed on the surface of T7 phage. The cry1Ac gene was fused to the C-terminal end of T7-10B capsid protein and displayed on the surface of T7 phage as revealed by Western blot analysis of the purified phage particles. The T7-Cry1Ac phages retained toxicity against Manduca sexta larvae. We demonstrated that the T7-Cry1Ac phage interacts with Cry1Ac receptors present in M. sexta BBMV either in solution or in overlay binding assays. (c) 2006 Elsevier Inc. All rights reserved.
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